Grand Seromics™ service details & data deliverables.
The typical HuProt™ microarray service involves case and control serum samples diluted 1:1000 and reacted with individual microarrays with BSA blocking. After incubation, samples are stained for one or two desired fluorescent secondary detection antibodies (ie IgG / IgA / IgM). Arrays are scanned and raw TIF images are created using Molecular Devices Genepix microarray scanners. Spots are then aligned to CDI library (.gal) files, and spot readings are output as raw Genepix results (.gpr) files. These raw results are then delivered both as .gpr files and combined data tables. If the samples are blinded to CDI, this is the final output. If sample blinding is not a requirement, CDI can additionally prepare basic quantile normalized case / control group statistics with ranked p-values from Student’s t-tests, Wilcoxon signed-rank tests, and other statistical outputs.
The typical HuScan™ & VirScan™ PhIP-Seq service involves case and control serum or plasma samples. These are heat deactivated and undergo a protein A/G pulldown assay, PCR amplification, and next-generation sequencing. Raw sequence data are run through a normalization and quantitation pipeline as previously described (Mohan et al., 2018). Raw pipeline outputs are then provided to customers as normalized read counts data tables for both whole proteins and individual peptides.