Enter new dimensions of antigen discovery.
With Grand Seromics™ service you’re able to get a combined readout from each of the core ANTYGEN™ platform services – integrating data from HuProt™ human proteome microarrays, HuScan™ human phage display immunoprecipitation sequencing, and VirScan™ human virome phage display immunoprecipitation sequencing — providing antigen-specific immunologic insights never before possible. This product is available as a service to both academic and industry customers.
Grand Seromics™ service details & data deliverables.
The typical HuProt™ microarray service involves case and control serum samples diluted 1:1000 and reacted with individual microarrays with BSA blocking. After incubation, samples are stained for one or two desired fluorescent secondary detection antibodies (ie IgG / IgA / IgM). Arrays are scanned and raw TIF images are created using Molecular Devices Genepix microarray scanners. Spots are then aligned to CDI library (.gal) files, and spot readings are output as raw Genepix results (.gpr) files. These raw results are then delivered both as .gpr files and combined data tables. If the samples are blinded to CDI, this is the final output. If sample blinding is not a requirement, CDI can additionally prepare basic quantile normalized case / control group statistics with ranked p-values from Student’s t-tests, Wilcoxon signed-rank tests, and other statistical outputs.
The typical HuScan™ & VirScan™ PhIP-Seq service involves case and control serum or plasma samples. These are heat deactivated and undergo a protein A/G pulldown assay, PCR amplification, and next-generation sequencing. Raw sequence data are run through a normalization and quantitation pipeline as previously described (Mohan et al., 2018). Raw pipeline outputs are then provided to customers as normalized read counts data tables for both whole proteins and individual peptides.
ANTYGEN Grand Seromics™ service sample requirements.
Human serum or plasma only: Send three 20μL aliquots per sample.
Sample shipping & sample return.
We will include shipping details in your quote – you must cover the cost of shipping samples to CDI. Typically, after you receive your report, CDI keeps the remaining samples for two months and then disposes of them. When shipping to us, please let us know if you want the remaining samples returned after the study is complete. We will charge for return shipping.