HuProt™: THE HUMAN PROTEOME MICROARRAY
The world’s largest collection of full-length human proteins.
There have been other protein microarrays, but none were made from a protein library as comprehensive or thoroughly validated as HuProt™. Created by faculty at the High Throughput Biology (HIT) Center at the Johns Hopkins University School of Medicine, HuProt was the brainchild of CDI co-founders Jef Boeke, Heng Zhu, Dan Eichinger, & Seth Blackshaw. Original development on HuProt was funded by the NIH Common Fund Support for the Development of Protein Capture Reagents and Technologies, a major project which resulted in HuProt-validated monospecific monoclonal antibodies for human transcription factors. These antibodies, along with many others, are now sold by CDI as Monomabs™.
The collection starts with sequence-confirmed plasmids, which are used to make >21,000 GST-purified recombinant proteins in yeast. After purification, the GST-tagged proteins are piezoelectrically printed on glass slides in duplicate, along with control proteins (GST, BSA, Histones, IgG, etc.). Slides are barcoded for tracking and archiving. Each microarray batch is routinely evaluated by anti-GST staining to demonstrate quality of expression and printing. Slides can be PATH™ nitrocellulose or SuperEpoxy2™. HuProt arrays have been used to evaluate DNA & RNA nucleotide binding, antibody specificity, small molecule binding, protein-protein interactions, and more to properly folded, three-dimensional human proteins.
Technology overview.

Next-generation technology. More content, cleaner data.
We start with sequence-confirmed plasmids, then individually express and GST-purify proteins from S. cerevisiae. Piezoelectric printing is used to spot these in duplicate alongside controls in batches of up to 1000 arrays; quality confirmed with anti-GST QA/QC. Successful folding demonstrated by kinase autophosphorylation assay. Service available.

Broad coverage of the human proteome.
The new HuProt v4.0 consists of >21,000 unique human proteins, isoform variants, and protein fragments – covering 16,794 unique genes. This includes 15,889 of the 19,613 canonical human proteins described in the Human Protein Atlas, with broad coverage across protein subclasses.
Content includes major functional classes such as intracellular proteins, membrane proteins, enzymes, secreted proteins, transcription factors, transporters, GPCRs, cytokines, immune receptors, immune checkpoints, CD markers, ion channels, cytosolic proteins, nuclear receptors. Additionally there is thorough coverage for proteins enriched in major tissues of interest such as testis, cerebral cortex, thyroid gland, skin, fallopian tube, liver, parathyroid, intestine, kidney, spleen, muscle, epididymis, lymph node, bone marrow, adrenal gland, esophagus, heart, appendix, tonsil, prostate, rectum, adipose tissue, stomach, colon, cervix, uterus, gallbladder, seminal vesicle, breast, ovary, endometrium, smooth muscle, salivary gland, pancreas, and bladder.

Reproducible protein distribution.
CDI’s non-contact piezoelectric ‘inkjet’ process uses next-generation ArrayJet printers and allows for rapid production of high quality microarray slides time after time. Versus older contact pin printing methods – HuProt™ arrays are made with improved accuracy and reproducibility with excellent spot morphology.

Reproducible serum profiling.
Reproducible Proteome-Wide IgG Autoantibody Immunoprofiling of a Healthy Human Male Within and Across HuProt Proteome Microarray Batches. Serum was collected from a healthy adult human male donor, incubated on pairs (Rep1, Rep2) of HuProt proteome microarrays across three print batches (Batch 1 Feb12_2020, Batch 2 Dec09_2019, Batch 3 Oct01_2019), and stained with anti-IgG (red) & anti-IgA (green) secondaries. Raw data were plotted on a log scale and linear regression analysis was performed. Intra-lot correlations of spot pair averages (red boxes) was >.95 R^2 within all three batches. Slide-to slide cross pairings across all possible pairs of the six slides was a >.90 R^2 correlation – demonstrating robust reproducibility of HuProt microarray data between any individual slide; these results demonstrate multi-isotype analysis requiring multiple slides should be reliable.


Learn more about our ANTYGEN™ HuProt™ analysis services.
Deep dive on HuProt™ proteome microarray technology and QA/QC.
HuProt™ Microarray Production. The HuProt™ Human Proteome Microarray is the most comprehensive human proteome array created to date (Jeong et al, 2012). It contains over 21,000 human proteins and protein isoforms, including >81% of canonically expressed proteins as defined by the Human Protein Atlas, and allows hundreds of interactions to be profiled in high-throughput. HuProt™ can be used for a wide range of applications-this includes mapping antigen-specific immunity as multi-isotype profiles in serum, determining monoclonal antibody specificity, and studying protein-protein interaction, substrate identification, protein-DNA binding, protein-RNA binding, and binding of some small molecules. CDI Labs’ latest version of the array, HuProt™ v4.0, contains >81% of human proteins in each major functional Gene Ontology protein category (Venkataraman et al, 2018).
Creation of HuProt™ Library. HuProt™ library clones were derived from public ORF libraries or independently synthesized; entry clones are from the laboratories of Heng Zhu and Seth Blackshaw (The Johns Hopkins University). Using the Gateway recombinant cloning system (Invitrogen, CA), human ORFs were shuttled from the entry clones to a yeast high-copy expression vector (pEGH-A) that produces GST-His6 fusion proteins under the control of the galactose-inducible GAL1 promoter. Plasmids were rescued into E. coli and verified by restriction endonuclease digestion. Plasmids with inserts of correct size were transformed into yeast for protein purification (Hu S et al, 2009; Jeong J et al, 2012)
Validating and Curating Clones used in HuProt™. To check and confirm the identity of each human ORF in the HuProt™ libary, bidirectional Sanger sequencing was conducted on both the entry clones and the yeast expression vectors that were derived from them (Venkataraman A et al, 2018). Blast+ was used to align the ORF sequence to multiple public databases (UniProt, CCDS, RefSeq, and Ensembl) to generate an integrated alignment score for each clone. If a clone covered the entire sequence of a known protein, the clone is considered full length (F), whereas partial matches were regarded as indicative of truncated (TRUNC) clones. Because the source clones included ORFs containing untranslated regions, unannotated splice variants, and single-nucleotide polymorphisms, the clones were categorized into groups ranging from perfect matches to the known protein-coding transcriptome, to as-yet potential protein-coding ORFs that are not yet reviewed. A detailed breakdown of this classification, along with the threshold parameters, can be accessed at https://collection.cdi-lab.com/public.
Protein Purification from the HuProt™ Library. Proteins were purified from yeast transformed with expression vectors encoding the human ORFs. Human proteins were purified as GST-His6 fusion proteins from yeast using a previously described high-throughput purification protocol (Hu S et al, 2009; Zhu et al., 2001). Using a 96-well format, the samples are purified from yeast extracts using glutathione-agarose beads. 0.1% Triton is included in the lysis buffer and washers to ensure that the purified proteins are free of lipids.
Protein Microarray Production & Testing. The purified human proteins were arrayed in a 384-well format and printed on PATH slides (GraceBio, USA), using an Arrayjet UltraMarathon printer (Arrayjet, UK) to create a block format. Arrays that show >95% of the spots with a foreground/background signal (F/B) ratio of at least 1.5 in an anti-GST assay are classified usable. A number of controls that are reactive with secondary detection reagents are included on HuProt™. Controls include titrated GST protein, histones, mouse and rabbit anti-biotin, mouse IgM, and biotin-tagged control for streptavidin detection. Each block also contains a row of control spots, including Alex Fluor 555/647 as landmarks.
Tests show that HuProt™ arrays contain a majority of the annotated, full-length proteome in native conformation. Tests on HuProt™ show that the proteins are folded in native conformation and retain function (Hu S. poster; Venkataraman A., et al., 2018). When both native and denatured HuProt™ arrays were probed with monoclonal antibodies that selectively recognize either linear or folded epitopes of their cognate antigen, the antibodies were found to recognize the appropriate antigen form (Venkataraman A., et al., 2018). Further tests (RNA binding) showed that proteins on HuProt™ do retain function (Venkataraman A., et al., 2018).
HuProt™ references.
Autoantibody Profiling
- Gruber C. et al (2020). Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell.
- Li Y. et al (2020). Longitudinal serum autoantibody repertoire proflining identifies surgery-associated biomakers in lung adenocarcinomas. EBioMedicine
- Pan, J. et al (2020). Integration of IgA and IgG Autoantigens Improves Performance of Biomarker Panels for Early Diagnosis of Lung Cancer. Mol Cell Proteomics. 2020 Mar;19(3):490-500.
- Osman, I. e al (2020). Using autoantibody signatures to predict immunotherapy discontinuation in melanoma patients. J. Clinical Oncology 38 (15).
- Longobardi, S. et al (2020). Novel shared antibody specificities in anti-Ro/ La antibody negative Sjögren’s Syndrome. J Immunol May 1, 2020, 204 (1 Supplement) 218.20
- Dhande, I. et al. (2020). Stim1 Polymorphism Disrupts Immune Signaling and Creates Renal Injury in Hypertension. Journal of the American Heart Association. 2020;9
- Zhang, S. et al. (2020) Autoantibody signature in hepatocellular carcinoma using seromics. Journal of Hematology & Oncology. 13 (85)
- Zaenker, P. et al. (2020). Tropomyosin autoantibodies associated with checkpoint inhibitor myositis. Oncoimmunology. 9(1)
- Ma, A. et al (2020). Serum Levels of Autoantibodies Against Extracellular Antigens and Neutrophil Granule Proteins Increase in Patients with COPD Compared to Non-COPD Smokers. International Journal of Chronic Obstructive Pulmonary Disease (15): 189—200.
- Kim, S. et al. (2020). B Cells Improve Overall Survival in HPV-Associated Squamous Cell Carcinomas and Are Activated by Radiation and PD-1 Blockade. Clin Cancer Res 2020;26:3345–59
- Rowley, A. et al. (2020). A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease. J. Infectious Diseases, 222(1):158–168
- Ling. H. et al. (2020). Discovery of new serum biomarker panels for systemic lupus erythematosus diagnosis. Rheumatology Volume 59, Issue 6, June 2020, Pages 1416–1425
- Dhande, I et al. (2019). Germ-line genetic variation in the immunoglobulin heavy chain creates stroke susceptibility in the spontaneously hypertensive rat. Physiological Genomics
- Zhao, T. et al. (2019). ANGPTL3 inhibits renal cell carcinoma metastasis by inhibiting VASP phosphorylation. Biochemical and Biophysical Research Communications. 516(3): 880-887
- Dhame, I. et al. (2019). Stim1 Polymorphism Disrupts Immune Signaling and Creates Renal Injury in Hypertension. JHMA 9(5). Journal of the American Heart Association. 2020;9
- Pan, J . Et al. (2019). Discovery and Validation of a Serologic Autoantibody Panel for Early Diagnosis of Esophageal Squamous Cell Carcinoma. Cancer Epidemiol Biomarkers Prev 2019;28:1454–60.
- Fan W et al. (2019) Sa1679–Multiple rather than specific Antibodies were identified in Irritable Bowel Syndrome using Huprot™ Microarray Approach Gastroenterology 156 (6), Supplement 1, Page S-364
- Lastwika KJ et al. (2019) Tumor-derived autoantibodies identify malignant pulmonary nodules. Am J Respir Crit Care Med, 199 (10)
- Ye S et al. (2019) Plasma proteomic and autoantibody profiles reveal the proteomic characteristics involved in longevity families in Bama, China Clin Proteomics,16:22
- Gowen MF et al. (2018) Baseline antibody profiles predict toxicity in melanoma patients treated with immune checkpoint inhibitors. J. Transl. Med., 16(1):82
- Bigley V et al. (2018) Biallelic interferon regulatory factor 8 mutation: A complex immunodeficiency syndrome with dendritic cell deficiency, monocytopenia, and immune dysregulation. J. Allergy Clin Immunol, 141(6):2234-2248
- Bremer HD et al. (2018) ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease Sci.Rep. 8(1):4852
- Chung JM et al. (2018) Identification of the thioredoxin-Like 2 Autoantibody as a Specific Biomarker for Triple-Negative Breast Cancer. J Breast Cancer 21:87-90
- Pan J et al. (2017) Identification of Serological Biomarkers for Early Diagnosis of Lung Cancer Using a Protein Array-Based Approach. Mol Cell Proteomics 16 (12):2069-2078
- Wang J et al. (2017) PTMA, a new identified autoantigen for oral submucous fibrosis, regulates oral submucous fibroblast proliferation and extracellular matrix. Oncotarget. 8(43): 74806–74819.
- Gupta S et al. (2017) Serum Profiling for Identification of Autoantibody Signatures in Diseases Using Protein Microarrays. Methods Mol Biol 1619:303-315.
- Gupta S et al. (2017) Evaluation of autoantibody signatures in meningioma patients using human proteome arrays. Oncotarget 8 (35): 58443-58456
- Chung et al. (2017) Phenotyping and auto-antibody production by liver-infiltrating B cells in primary sclerosing cholangitis and primary biliary cholangitis J. Autoimmun 77:45-54
- Ogishi et al. (2016) Delineation of autoantibody repertoire through differential proteogenomics in hepatitis C virus-induced cryoglobulinemia. Sci Rep 6:29532.
- Yang L et al. (2016) Identification of serum biomarkers for gastric cancer diagnosis using a human proteome microarray. Mol Cell Proteomics 15(2):614-23.
- Fiorentino DF et al. (2016) PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren’s syndrome. Ann Rheum Dis 75(6): 1145–1151.
- Hu CJ et al. (2016) Identification of Novel Biomarkers for Behcet Disease Diagnosis Using HuProt Array Approach. Mol Cell Proteomics 16(2):147-156
- Shi L et al. (2016) Application of high-throughput protein array in clinical screening for tumor markers. Int J Clin Exp Med 9:8529-8535.
- Syed P et al. (2015) Autoantibody profiling of glioma serum samples to identify biomarkers using human proteome arrays Sci Rep 15(5):13895
- Hu C et al. (2015) Autoantibody profiling on human proteome microarray for biomarker discovery in cerebrospinal fluid and sera of neuropsychiatric lupus. PLoS One 10(5):e0126643
- Hu CJ et al. (2012). Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays. Mol Cell Proteomics 11(9):669-80.
Antibody Specificity and Crossreactivity
- Kim, Y et al (2019). Renoprotective effects of a novel cMet agonistic antibody on kidney fibrosis. Scientific Reports , Volume 9, Article number: 13495
- Venkataraman A et al. (2018) A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nat Methods , in press
- Ramos-López et al. (2018) Antibody Specificity Profiling using Protein Microarrays. In: Rockberg J., Nilvebrant J. (eds) Epitope Mapping Protocols, Methods in Molecular Biology, vol 1785. e0181251.
- Washburn N et al. (2017) High-resolution physicochemical characterization of different intravenous immunoglobulin products. PLoS One 2017 Jul 31
- Sterner E et al. (2017) Therapeutic Antibodies to Ganglioside GD2 Evolved from Highly Selective Germline Antibodies. Cell Rep 20(7):1681-1691
- Kim YP et al (2014) Effective Therapeutic Approach for Head and Neck Cancer by an Engineered Minibody Targeting the EGFR Receptor. PLoS One. 1;9(12):e113442.
- Liu S et al. (2014) Characterization of monoclonal antibody’s binding kinetics using oblique-incidence reflectivity difference approach. MAbs 7:110-119
- Jeong JS et al. (2012) Rapid identification of monospecific monoclonal antibodies using a human proteome microarray. Mol Cell Proteomics 11(6):O111.016253
Protein Interactomics
- Kim, S. et al. (2020). Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 2020, 9(3), 595.
- Lucia, C. et al. (2020). Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein. Scientific Reports 10:1609
- Yue, H. et al. (2019). Calpastatin participates in the regulation of cell migration in BAP1-deficient uveal melanoma cells. Int J Ophthalmol. 2019; 12(11): 1680–1687.
- Song, G. et al. (2020). An Integrated Systems Biology Approach Identifies the Proteasome as a Critical Host Machinery for ZIKV and DENV Replication. DOI: 10.1101/2020.03.04.976548
- Yu JJ. et al. (2020). TRIB3-EGFR interaction promotes lung cancer progression and defines a therapeutic target. Nature Communications volume 11, Article number: 3660 (2020
- Zhao T et al. (2019) DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma Theranostics; 9(21): 6175–6190
- Song G et al. (2019) Proteome-wide Tyrosine Phosphorylation Analysis Reveals Dysregulated Signaling Pathways in Ovarian Tumors. Mol Cell Proteomics. 18(3):448-460.
- Cao T et al. (2019) A Two-Way Proteome Microarray Strategy to Identify Novel Mycobacterium tuberculosis-Human Interactors. Front. Cell. Infect. Microbiol, 28;9:65
- Cho HM et al. (2019) Drp1-Zip1 Interaction Regulates Mitochondrial Quality Surveillance System Mol Cell. 73(2):364-376.e8
- Wu FL et al. (2018) Global profiling of PknG interactions using a human proteome microarray reveals novel connections with CypA. Proteomics, Dec;18(23):e1800265
- Feng Y et al. (2018) High-Throughput Chip Assay for Investigating Escherichia coli Interaction with the Blood-Brain Barrier Using Microbial and Human Proteome Microarrays (Dual-Microarray Technology) Anal Chem 90(18):10958-10966
- Guo G et al. (2018) The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation. Cell Death Dis 24(9):2
- Choi S et al (2018) PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Mol Cell. 70(5):920-935.e7.
- Bao Y et al (2018) Angiopoietin-like protein 3 blocks nuclear import of FAK and contributes to sorafenib response. Br J Cancer. 119(4):450-461.
- Yang Z et al. (2017) A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18. Mol Cell Proteomics 16:469-484
- Cox E et al. (2017) Global Analysis of SUMO-Binding Proteins Identifies SUMOylation as a Key Regulator of the INO80 Chromatin Remodeling Complex. Mol Cell Proteomics 16:812-23
- Liu M et al. (2017) Disruption of Ssp411 causes impaired sperm head formation and male sterility in mice. Biochim Biophys Acta Gen Subj. 1862(3):660-668.
- Li H et al. (2016) Penetrance of Congenital Heart Disease in a Mouse Model of Down Syndrome Depends on a Trisomic Potentiator of a Disomic Modifier. Genetics 203(2):763-70
- Wang Y et al. (2016) A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1. Science 354 (6308)
- Ainscough J et al. (2015) Interleukin-1β Processing Is Dependent on a Calcium-mediated Interaction with Calmodulin. J. Biol. Chem. 290(52):31151-61
- Hu J et al. (2015) Systematic prediction of scaffold proteins reveals new design principles in scaffold-mediated signal transduction. PLoS Comput Biol 11(9):e1004508
- Jung JG et al. (2014) Notch3 interactome analysis identified WWP2 as a negative regulator of Notch3 signaling in ovarian cancer. PLos Genet10:e1004751.
- Ma TM et al. (2014) Serine racemase regulated by binding to stargazin and PSD-95: Potential NMDA-AMPA glutamate neurotransmission cross-talk. J Biol Chem. 289(43):29631-41
- Deng, RP et al. (2014) Global Identification of O-GlcNAc Transferase (OGT) Interactors by a Human Proteome Microarray and the Construction of an OGT Interactome. Proteomics 14:1020-30.
- Fan, Q et al. (2014) Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. Mol Vis 20:117-124.
- Chen Y et al. (2013) Bcl2-associated Athanogene 3 Interactome Analysis Reveals a New Role in Modulating Proteasome Activity. Mol Cell Proteomics 12(10):2804-19.
- Huang Y. et al (2012) Global tumor protein p53/p63 interactome: making a case for cisplatin chemoresistance. Cell Cycle 11(12):2367-79.
Kinase assays
- Hu J et al. (2015) Systematic prediction of scaffold proteins reveals new design principles in scaffold-mediated signal transduction. PLoS Comput Biol 11(9):e1004508
- Tarrant MK et al. (2012) Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis. Nat Chem Biol 8(3):262-9.
Other enzyme assays
- Crawford, L. et al. (2020). The E3 ligase HUWE1 inhibition as a therapeutic strategy to target MYC in multiple myeloma. Oncogene volume 39, pages 5001–5014
- Choi, S. et al. (2018). PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Molecular Cell Volume 70, Issue 5, 7 June 2018, Pages 920-935.e7
- Uzoma I et al. (2018) Global Identification of Small Ubiquitin-related Modifier (SUMO) Substrates Reveals Crosstalk between SUMOylation and Phosphorylation Promotes Cell Migration. Mol. Cell. Proteomics 17(5):871-888
- Xu Z et al. (2017) Systematic identification of the protein substrates of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T1/T2/T3 using a human proteome microarray. Proteomics 17:11
- Cox E et al. (2015) Identification of SUMO E3 ligase-specific substrates using the HuProt human proteome microarray. Methods Mol Biol. 1295:455-63
- Lee YI et al. (2013) Protein microarray characterization of the S-nitrosoproteome. Mol Cell Proteomics 13:63-72.
Nucleic Acid Binding
- Yu, B. et al (2020). A Novel LncRNA, AC091729.7 Promotes Sinonasal Squamous Cell Carcinomas Proliferation and Invasion Through Binding SRSF2 Front Oncol. 2019; 9: 1575.doi: 10.3389/fonc.2019.01575
- Panatta. E. et a. (2020). Long non-coding RNA uc.291 controls epithelial differentiation by interfering with the ACTL6A/BAF complex. EMBO Reports e46734
- Wang, Q. et al (2020). The Rs12569232 SNP Association with Vogt-Koyanagi-Harada Disease and Behcet’s Disease is Probably Mediated by Regulation of Linc00467 Expression. Ocular Immunology and Inflammation
- Kang CL et al. (2019) LncRNA AY promotes hepatocellular carcinoma metastasis by stimulating ITGAV transcription Theranostics 9(15):4421-4436
- Liu L et al. (2019) Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome. Nucleic Acid Res. 47(5):2244-2262
- McClymont et al. (2018) Parkinson-Associated SNCA Enhancer Variants Revealed by Open Chromatin in Mouse Dopamine Neurons. Am J Hum Genet 103(6):874-892
- Qin M et al. (2018) Circ-UBR5: An exonic circular RNA and novel small nuclear RNA involved in RNA splicing. Biochem Biophys Res Comm 503 (2):1027-1034.
- Wang Y et al. (2018) HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest. 128(12):5235-5250.
- Barry G et al. (2017) The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states. Sci Rep 7:40127
- Barry G et al. (2014) The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing. Molec Psychiatry. 19(4):486-94.
- Donnelly CJ et al. (2013) RNA toxicity from the ALS/FTD C9ORF72 expansion is mitigated by antisense intervention. Neuron 80(2):415-28.
- Fan B et al. (2013) A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5′ terminal sequence of the hepatitis C virus RNA. Mol Cell Proteomics. 13(1):84-92.
- Hu S. et al (2009) Profiling the human protein-DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell. 139(3):610-22.
Small molecule profiling
- Chen X. et al (2020). Celastrol induces ROS-mediated apoptosis via directly targeting peroxiredoxin-2 in gastric cancer cells. Theranostics 2020; 10(22):10290-10308.
- Chen, P. et al (2020). High-throughput screening suggests glutathione synthetase as an anti-tumor target of polydatin using human proteome chip. International Journal of Biological Macromolecules Volume 161, 15 October 2020, Pages 1230-1239.
- Dai, X et al (2018). Osthole inhibits triple negative breast cancer cells by suppressing STAT3. Journal of Experimental & Clinical Cancer Research Volume 37, Article number: 322 (2018)
- Jia D et al (2019) Cardioprotective mechanism study of salvianic acid A sodium based on a proteome microarray approach and metabolomic profiling of rat serum Mol. Omics 2019 15(4):271-279.
- Zhang HN et al (2015) Systematic identification of arsenic-binding proteins reveals that hexokinase-2 is inhibited by arsenic. Proc Natl Acad Sci U.S.A. 112 (49):15084-9
Other
- Garranzo-Asensio, M. et a. (2020). Protein Microarrays: Valuable Tools for Ocular Diseases Research. Current Medicinal Chemistry, Volume 27, Number 27, 2020, pp. 4549-4566(18)
- Qi, H. et al (2019). Proteome microarray technology and application: higher, wider, and deeper. Expert Review of Proteomics 16 (10): Pages 815-827. https://doi.org/10.1080/14789450.2019.1662303
- Chen, J et al (2019) Protein domain microarrays as a platform to decipher signaling pathways and the histone code. Methods, 2019 – Elsevier
- Gupta, S. & S. Srivastava (2019). Chapter 10 – Prospects of translational proteomics and protein microarrays in oligodendroglioma.
- Syu GD (2019) Development and application of a high-content virion display human GPCR array. Nature Communications 10: 1997
- Coll JM (2018) Herpesvirus infection induces both specific and heterologous antiviral antibodies in carp. Front Immunol. 9 (39) Jan 2018
- Azevedo C (2018) Screening a Protein Array with synthetic biotinylated Inorganic Polyphosphate to define the human PolyP-ome.ACS Chem Biol. 13 (8):1958-1963