PHAGE DISPLAY IMMUNOPRECIPITATION  SEQUENCING: PhIP-Seq

The Story of PhIP-Seq — Antigen Library Phage Display, Immunoprecipitation, and Sequencing

Bacteriophage Immunoprecipitation Sequencing (PhIP-Seq) is a powerful new method of multiplexed analysis that combines DNA high-throughput sequencing with next-generation proteomics. PhIP-Seq allows researchers to determine what antibodies were created by an individual’s unique history of immune exposures. Our PhIP-Seq assay is the highest-coverage antigen-specific assay ever developed, able to detect antibodies versus every known human protein or virus as overlapping long peptides.

PhIP-Seq was developed at Harvard University by CDI Scientific Advisory Board members Stephen Elledge, Ben Larman and colleagues in 2011. This work created HuScan™, a synthetic representation of the complete human proteome as a T7 bacteriophage display library. They built upon this work in 2015, by creating VirScan™, a synthetic representation of the complete human virome: the universe of known infectious viruses.

Since then, PhIP-Seq has been used to perform revolutionary immune profiling on people suffering from measles, multiple sclerosis, diabetes, rheumatoid arthritis, and more.  By sequencing millions of DNA strands at once, CDI’s PhIP-Seq is a powerful technology that  helps scientists determine the immune system’s role in complex disease non-invasively, quickly, and at a low cost.

Technology Overview

PhIP-Seq in the News

PhIP-Seq: HuScan™ — Human proteome phage display

Human proteome epitope-level autoantibody profiling service via T7 phage display and immunonprecipitation sequencing. The entire UniProt human proteome — in a single assay.

Learn more about our ANTYGEN™ HuScan™ analysis services standalone or as part of a Grand Seromics assay:

HuScan: Immune Profiling Literature Citations

Mohan D et al. (2018) PhIP-Seq characterization of serum antibodies using oligonucleotide-encoded peptidomes. Nat Protoc 3:1958-1978.

Larman et al.. (2013) PhIP-Seq characterization of autoantibodies from patients with multiple sclerosis, type 1 diabetes and rheumatoid arthritis. J Autoimmun 43:1-9.

Larman et al. (2011) Autoantigen discovery with a synthetic human peptidome. Nat Biotechnol 29:535–541.

PhIP-Seq: VirScan™ — Human virome phage display

Human virome epitope-level antiviral antibody profiling service via T7 phage display and immunonprecipitation sequencing. The entire UniProt human virome — in a single assay.

Learn more about our ANTYGEN™ VirScan™ analysis services standalone or as part of a Grand Seromics assay:

VirScan: Immune Profiling Literature Citations

Xu GJ et al. (2015) Viral immunology. Comprehensive serological profiling of human populations using a synthetic human virome. Science 348:(6239):aaa0698.

Isnard P et al. (2019) Temporal virus serological profiling of kidney graft recipients using VirScan. Proc Natl Acad Sci USA 116:10899-10904.